Engineering of high-precision base editors for site-specific single nucleotide replacement
نویسندگان
چکیده
منابع مشابه
A Microfluidic Device for Detection of Single Nucleotide Polymorphisms by Allele Specific Single Base Extension
We present a microfluidic device with integrated temperature sensors and heaters for single nucleotide polymorphism (SNP) detection. The allele specific primer is immediately annealed next to the polymorphic site, and extended by a single base. The extension product is then purified by solid phase capture, and subsequently released via chemical cleavage. After desalting, the released product is...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2019
ISSN: 2041-1723
DOI: 10.1038/s41467-018-08034-8